Shoot a gray card, out of focus, at maximum aperture value (smallest hole).
Or a lightbox (underexposed to be gray) out of focus with maximum aperture value.
Then in Capture One, Lightroom, or Photoshop increase the contrast or otherwise exaggerate the histogram.
Best to clean your digital sensor first to make sure you don't confuse sensor dust with lens dust/fungus.
If you're shooting film... I don't know... borrow a digital back :-).
Doug Peterson
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Head of Technical Services, Capture Integration
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