Is it normal to get quite different results from one measurement to the other?
Meaning, if you are reading the same patches, no.
Does ambient light have an influence on the measurements?
Meaning, ambient light while you are reading the patches, no. But be aware that if the paper is thin-ish, you should put some white paper underneath before reading.
Your lighting will make quite a difference when looking at the test prints. I look at them in similar light to the one where they will be displayed.
Should I now set 10 to be absolute black.
You can probably tweak a little more out of the shadows by opening the profile, and under "SpyderProof-View" select "advanced editing" at the bottom, select the advanced tab and move the shadow detail to +5. Save as a separate profile.
Most of canned profiles for the papers I use don't give any separation in the blacks under 8. If there is important shadow detail under about 50 or so, I open up shadow detail and minimise black clipping in the raw conversion if possible. Then in Photoshop I set my significant blacks to no lower than 8.
I guess in LR you could do this by holding down the alt key and moving the black slider until clipped blacks show up, send the slider back to where it was and drop the mouse over that spot and read the value under the histogram. Multiply by 2.5 to get an approximation of that value for a scale of 0-255 (instead of Lightroom's 0-100).
BTW, for a glossy paper I would make sure there is nothing being printed over a value of 251 or so, as there will be no ink being laid down and you can see it in the final print.
Or is this the same as using relative as a rendering intent?
No. Choose your rendering intent when softproofing in Lightroom
Cheers, David
Edit: I've just done a profile for the new Epson Legacy Baryta with the Spyderprint SR for my Canon 6300 printer, and am quite pleased with the result. I use the plastic strip reader to hold the spectro still and centre it on each patch, but don't use the actual strip reading function as I'm not confident it's reliable. 729 patches sound a lot to read but doesn't take that long.