Luminous Landscape Forum

Equipment & Techniques => Medium Format / Film / Digital Backs – and Large Sensor Photography => Topic started by: Justinr on January 12, 2014, 01:25:26 pm

Title: MF Macro examples
Post by: Justinr on January 12, 2014, 01:25:26 pm
Has anybody done much in the way of macro work with an MF and do they have some results online to compare and contrast?  I've been messing around all day trying to get some decent images from the Mamiya/ZD with 120mm macro  lens and have not achieved anything that I am happy with. In fact this is the best I managed and it's just too soft to my mind. I'm trying to work out if it's me or the camera or whether I'm expecting too much.

Title: Re: MF Macro examples
Post by: Hank Keeton on January 12, 2014, 02:36:06 pm
Yes....I do lots.....largely with Hassy-V + Aptus-65, but I also use movements with my Sinar P2. My website has numerous examples. I commonly use Helicon-Focus to achieve the DoF I want...but movements also allow me to expand those optical limits. Natural lighting is a must....but I will sometimes augment that, to highlight certain features or provide a different "look."

It's a great compliment to my WA or tele work in general....

Cheers!
Title: Re: MF Macro examples
Post by: amsp on January 12, 2014, 02:40:18 pm
The Mamiya 120mm Macro is a stellar and painfully sharp lens, so there's something wrong either with your lens, camera or technique.
Title: Re: MF Macro examples
Post by: Brian Hirschfeld on January 12, 2014, 04:29:26 pm
Yes, I agree, the 120mm f/4 Macro is a great lens, probably one of the best for the system. why don't you walk us through your shooting technique from the ground up.
Title: Re: MF Macro examples
Post by: Justinr on January 13, 2014, 03:49:44 am
Yes, I agree, the 120mm f/4 Macro is a great lens, probably one of the best for the system. why don't you walk us through your shooting technique from the ground up.

I've attached a picture of the set up being used. The Mamiya is mounted on a tripod with a synch cable to the overhead flash unit, the second flash unit is on slave.

The subject is placed in a light tent as seen with a third small flash head situated down behind reflecting off the screen, so there is plenty of diffused light. The overhead lamp is the main source whilst I use a snoot on the fill to direct light at a slight angle across the subject to give the image some lift.

The photo of the lichen above was taken at 1/80th, f32, ISO50 and was the sharpest I had obtained.

I bring the camera as close to the subject as the lens will focus then back off an inch or two so I haven't got it pinned at its minimum focusing distance.

I have tried focusing both by eye and the manual focusing aid.

 
Title: Re: MF Macro examples
Post by: synn on January 13, 2014, 04:01:12 am
Are you using mirror lockup and a remote shutter release?
Title: Re: MF Macro examples
Post by: Justinr on January 13, 2014, 04:09:17 am
Remote release yes, mirror lock up no, that could be worth a try.
Title: Re: MF Macro examples
Post by: synn on January 13, 2014, 04:11:26 am
f32 can't be helping. Mirror up is essential.

Yep, that too.
Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 13, 2014, 05:53:58 am
f32 can't be helping.

That's putting it mildly ...

It will utterly kill all micro-contrast and significantly reduce overall resolution, is closer to what it does.

The f/32 aperture will produce a diffraction blur pattern with a 44 micron diameter, covering a huge number of sensels, and that's not even including residual lens aberrations and defocus (DOF is very shallow in photomacrography) blur. Diffraction alone will physically limit resolution to 55.4 cycles/mm for average luminance, equivalent to some 70% of the maximum resolution of a 6.4 micron pitch sensor array. Again, that's only the optical diffraction component, actual resolution will be even less.

Cheers,
Bart
Title: Re: MF Macro examples
Post by: Justinr on January 13, 2014, 06:06:27 am
First of all many thanks for the input folk, it is appreciated.

I've just done some jewellery with the mirror locked up and the aperture at f29 and it does seem a lot better. I'll have another go at the lichen later for a true comparison but I think the mirror was the major problem. I had pushed the aperture to the limit in a vain attempt to gain sharpness so I'll be follow your advice Bart and try backing off that as well.

Have since given my forehead a good slap!  :D
Title: Re: MF Macro examples
Post by: yaya on January 13, 2014, 07:03:26 am
With your 9 micron sensor and the 120mm you can get decent results at f16 or even f22 with a bit of sharpening. If you need more DOF I suggest that you use Focus stacking
Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 13, 2014, 08:03:01 am
With your 9 micron sensor and the 120mm you can get decent results at f16 or even f22 with a bit of sharpening. If you need more DOF I suggest that you use Focus stacking

Indeed.

With a 9 micron pitch sensor that will work out somewhat better than for smaller sensel pitches, but given the close-up nature of the subjects, it would be safer to stay at f/16 - f/18 as narrowest apertures. Depending on the actual magnification factor, that will give a DOF of about 3.5 millimetres, so stacking will very quickly become the only option for improved DOF and sharpness. This all assumes no down-sampling for output.

Cheers,
Bart
Title: Re: MF Macro examples
Post by: Brian Hirschfeld on January 13, 2014, 08:58:55 am
Yes as others have said. There are a number of issues.

1.) f/32 is absolutely killing you. You'll be good up up to f/11 and possibly f/16 and then once your past f/22 diffraction is going to be absolutely killing any chance you could hope for of a sharp image.
2.) Selftimer for 5 seconds or so to reduce vibrations.
3.) mirror lock up further reduce vibrations
4.) cable release or tethered triggering with C1 or whatever.
5.) If you can bump the exposure up to 1/125 or something you might remove the risk of environmental factors causing vibration I.e people walking (including yourself) or house hold equipment. Etc.
6.) extended center column also destabilizes the tripod.

And yes as others have said, you might want to look into focus stacking. That might help you out. Like a lot actually. I know there's lots of solutions out there but I learned about focus stacking mostly from the Helicon Focus website. Can't speak to their product. Never used it for macro.
Title: Re: MF Macro examples
Post by: jerome_m on January 13, 2014, 12:14:46 pm
Has anybody done much in the way of macro work with an MF and do they have some results online to compare and contrast?

Ghislain Simard (http://www.simpho.com) is known for his insects photographed with an Hasselblad camera.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 13, 2014, 12:40:58 pm
Hi,

I would agree with most things said.

I have shot some samples for another reader on LuLa forums, they are all here: http://echophoto.dnsalias.net/ekr/Articles/MacroDemo_res/

The folder contains two layered tiff shot at f/8, f/16 and f/32 with a P45+ and a Zeiss Macro Planar 120/4 and also a Sony Alpha 99SLT and a Minolta 100/2.8 Macro. Bottom layer is f/32.

I also included two JPEG images that were stacked with Zerene Stacker, one from each camera, P45+ was shot at f/11 and Sony Alpha at f/8.

I also tried stacking with my very old copy of Helicon Focus but that did not work out well.

Best regards
Eik


Has anybody done much in the way of macro work with an MF and do they have some results online to compare and contrast?  I've been messing around all day trying to get some decent images from the Mamiya/ZD with 120mm macro  lens and have not achieved anything that I am happy with. In fact this is the best I managed and it's just too soft to my mind. I'm trying to work out if it's me or the camera or whether I'm expecting too much.


Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 12:50:18 am
When I had my Mamiya DF body and Aptus 12, I did a little test to measure useable DOF and also diffraction loss.   Here's a series of images from aperture f/5.7 to f/23 
Comparing these images to what you have, I'm wonder if you have more than just f/32 diffraction losses going on. 
btw - I think the 120 for mamiya is a good lens, but not the equal of the Rollei/Schneider 90mm APO macro or 150mm APO Macro.

First here is the full scene, followed by a crop of f/5.7, f/8, and f/13

 
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 12:54:05 am
and crops from f/16, f/18/, f/20, f/23

and this is with a 80mp back with a 5.2 um sensor. I'd say with a 9um sensor you'd see diffraction one to one and half stops more closed down from these I am posting.

Erik K. Do you best to control yourself.  I'm not going anywhere else with this discussion other than posting these images.
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 01:04:59 am
Here's a macro test shot from the Rollei 150mm macro at f/14 with the AFi-ii 12
The DOF is pretty thin here so I can see why you wanted to stop down more, but you really can't. Focus stacking as Yair suggested may be useful.  But I think you could go to f/20 with the 9um without taking too much of a hit and f/18 with the 5um backs, even further if your print size isn't large - as no one will notice.   

Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 01:27:34 am
I forgot to mention that in the mamiya 120 + Aptus 12 shots I posted the acceptable DOF appears to about 5 inches (again depending on print size / viewing distance) but my set up was further back from the bill than in your case.  You might try back up a bit and then using f/16 to catch the whole of your subject in DOF.   Even if you have to crop away a majority of your image, you may still have a sharper image with more of the subject within the DOF.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 14, 2014, 01:37:37 am
Hi Eric,

I don't like your attitude in this case. The OP asked for macro samples and I posted macro samples showing a three D subject at three different apertures with tow different camera systems, I also posted the same subject with the two cameras using focus stacking, using Zerene stacker. So I am giving the OP what he asked for.

I don't think you checked the images I posted. You are just assuming that they contradict your views, which I don't think they do.

As it may be you and I have different views and different experience, but I would say that my views may be as valid as yours.

I also have two issues with the test shots you have posted. One is that those images are in no way macro, an $ bill is no good way to test a macro shot as it does not have detail fine enough, the other is that at macro the actual aperture is decreased, due to magnification. At 1:1 macro it is usually 2 stops, so f/16 is no f/32. My own shots take this into account (in part), as they are close ups. That said, I feel that f/16 holds
up pretty well.

As I shot a 3D image the OP can judge the effect of DoF and diffraction, which cannot be done from your shots. I enclose an actual crop from the P45+ f/16 image, which I feel shows very good sharpness although being stopped down. What is wrong with that?

So I don't really understand your attitude.

Best regards
Erik

Erik K. Do you best to control yourself.  I'm not going anywhere else with this discussion other than posting these images.
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 01:40:35 am
Erik
I knew you couldn't resist!   ;D  
but wished you would have! 

btw - the 2 dollar bill in my image is at 45 degree angle to the lens axis - but you saw that right?  That's also why I have the ruler there - so I can quantitatively see the DOF.  My image is as 3D as needed.

My images show clearly diffraction losses with the 120, and they aren't as bad as you might guess.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 14, 2014, 01:51:33 am
Hi Eric,

Yes but your 2$ bill is not anywhere close to macro.

I was a bit surprised that my macro images held up so well at f/16. But I was also surprised how little DoF it would give on a true macro shot (I guess mine is around 1:3, still a bit from true macro).

But yes, I admit that I just glanced at your images. On the other hand I don't think you have checked mine, else I don't understand while you would comment as you did.

Getting back to the question the OP asked, I would say what is needed is exact focus, elimination of vibration. From the images I posted I would say that f/16 is OK f/32 I don't think so. If more DoF is needed I would try stacking.

I added two images, on left P45+ at f/32 and right P45+ at f/11 and stacking, full image and actual crops. Unstacked image on left, stacked on right.

Best regards
Erik

Erik
I knew you couldn't resist!   ;D  
but wished you would have!  

btw - the 2 dollar bill in my image is at 45 degree angle to the lens axis - but you saw that right?  That's also why I have the ruler there - so I can quantitatively see the DOF.  My image is as 3D as needed.

My images show clearly diffraction losses with the 120, and they aren't as bad as you might guess.
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 02:02:05 am
Yes, Erik,  my suggestion sometimes is to back away so you can get the whole subject in DOF even if cropping is required in post. It gets the job done whatever you want to call it. More of a practical tip.

Btw - this is the type of photography that multishot backs excel at.   Static subject needing DOF.   My tests show that you can stop down two full stops more with multi-shot than you can with singles shot.

ps. no never saw your images ( I only look at images taken with Rolleiflex cameras - just kidding! )  but every time I have posted images you make 4 or 5 posts about unrelated IQ issues.  I expected you to be pointing out the moire or find some false detail or something like that you've been digging into lately. More of a joke really since you are all over this forum.  
Title: Re: MF Macro examples
Post by: Justinr on January 14, 2014, 07:57:50 am
Phew!

Well I can certainly see a few areas of where I was going wrong and heartfelt thanks to the all who have suggested where matters might be improved. The question of aperture had never occurred to me as in my innocence I was thinking that the smaller the aperture the better the DOF without even realising that this created another set of problems with a digital sensor.

As yet I haven't had the time to put it into practice but seeing the results that can be achieved with photo stacking, plus improved technique and some purchase of appropriate software I think I will be getting a lot closer to what I want to achieve.
Title: Re: MF Macro examples
Post by: Doug Peterson on January 14, 2014, 09:48:29 am
Well I can certainly see a few areas of where I was going wrong and heartfelt thanks to the all who have suggested where matters might be improved. The question of aperture had never occurred to me as in my innocence I was thinking that the smaller the aperture the better the DOF without even realising that this created another set of problems with a digital sensor.

Diffraction softness due to too small an aperture opening affects film - it's not limited to digital sensors.

Digital sensors do make evaluation of sharpness against a known standard ("crisp at 100% pixel magnification") easier. Thereby it is easier to find that you have an issue with diffraction.

If you shot a 4x5 at f/64 and compared it side by side with a strong loupe to (or large print of) the same image at f/22 you'd find the same softening of fine detail.

There are many image-quality issues that digital makes easier to find. In my opinion there are two equally important lessons to take from that:
1) To be technically perfect on a medium requires greater skill/effort/control as you increase the quality of the medium.
2) The aesthetic, business, emotional, practical value of an image do not necessarily depend on technical perfection. Part of film's gift was that there was less "pixel-peeping" (or in this case "high-magnification loupe analysis").

Don't get be wrong on #2; I work as a technician at a high-end camera dealer because I understand and appreciate the nuance (and minutia) of the technical side of imaging. There are several genres where technical perfection is often a prerequisite to the image's efficacy: grand landscape, architecture, product/still-life, medical/scientific, art-reproduction, cultural heritage imaging (http://www.dtdch.com/), film scanning, aerial and many more. In such genres the raw capture nearly always needs to be sharp, well exposed, and properly color managed ("proper" might include a non-neutral WB). It goes without saying that these technical merits are only the pre-requisite; the image must also be visually compelling, tell a story etc.

In other genres the image itself does not necessarily need to exhibit any technical perfection to be an effective image ("effective" could mean visually appealing, monetarily successful, making it's creator personally proud or anything else). But even in these genres having a good understanding of the technical "rules" of photography make it easier to control/guide the creation of the image, and to break the rules with purpose rather than depending entirely on happy accidents.
Title: Re: MF Macro examples
Post by: Wim van Velzen on January 14, 2014, 01:44:16 pm
I recently posted some macro work here (http://www.luminous-landscape.com/forum/index.php?topic=85667.0). Plants taken with my Rollei and 90mm and 120mm lenses.

MLU and a sturdy tripod were necessary and even then I take  second or even third shot to be sure not some accidental movement caused blurring. In other words, the MF macro path isn't easy but in my eyes the rewards can be great.
Title: Re: MF Macro examples
Post by: Justinr on January 14, 2014, 01:58:21 pm
Progress so far -

Taken at 100th, f16, iso50 with mirror up and the camera on timer mode to allow it all to settle before shooting.

On my screen the magnification is about X10.
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 02:24:08 pm
Justinr,
Looks better definitely but I'm still wondering if you can't get even sharper.  I posted the 2 dollar bill examples to show what sharpness is possible with the 120mm lens, and how aperture settings will affect it.  I often will use a small flash for macro work positioned close to the subject as the flash duration is quite short with the small flash units - much shorter than with a studio type strobe. With a flash speed of 1/5000 or faster you don't have to worry much about vibration or movement.  If you can eliminate the vibration and movement, and have the aperture and focus set well, then what's left to check is the lens itself.  But DOF will be very small when you are focusing up that close, perhaps only a centimeter.
Title: Re: MF Macro examples
Post by: Justinr on January 14, 2014, 02:42:53 pm
Justinr,
Looks better definitely but I'm still wondering if you can't get even sharper.  I posted the 2 dollar bill examples to show what sharpness is possible with the 120mm lens, and how aperture settings will affect it.  I often will use a small flash for macro work positioned close to the subject as the flash duration is quite short with the small flash units - much shorter than with a studio type strobe. With a flash speed of 1/5000 or faster you don't have to worry much about vibration or movement.  If you can eliminate the vibration and movement, and have the aperture and focus set well, then what's left to check is the lens itself.  But DOF will be very small when you are focusing up that close, perhaps only a centimeter.


Two items that spring to mind are the UV filter on the lens and the IR filter over the sensor. Could they be making the difference?
Title: Re: MF Macro examples
Post by: EricWHiss on January 14, 2014, 03:10:02 pm
I wouldn't expect either of those to cause you problems. 
Do you get sharper images with the camera & back with other lenses?
Title: Re: MF Macro examples
Post by: Justinr on January 15, 2014, 05:35:46 am
I wouldn't expect either of those to cause you problems. 
Do you get sharper images with the camera & back with other lenses?

It would appear so yes.

This was taken with the 80mm standard lens -



Title: Re: MF Macro examples
Post by: Dragomir Spassov on January 15, 2014, 06:18:04 am
These also looks soft for me. Are they with applied sharpening?
Title: Re: MF Macro examples
Post by: Justinr on January 15, 2014, 08:50:00 am
This particular shot was focused manually as I wasn't too sure as to what auto focus was latching on to. The subject appears quite small in the viewfinder and trying to focus on an object (one of the circular structures) of about 1mm in width from a distance of two feet through an 80mm lens is a challenge to say the least, so yes I might have missed the desired focal plane but the AF shot (not shown) was no better. 

I have not applied any sharpening but upon further enlargement the structures appear to have as clear, if not clearer, delineation with the 80mm lens.
Title: Re: MF Macro examples
Post by: synn on January 15, 2014, 08:55:13 am
Why don't you try tethered shooting to ensure precise manual focus?
Title: Re: MF Macro examples
Post by: Justinr on January 15, 2014, 10:58:49 am
Why don't you try tethered shooting to ensure precise manual focus?

Two reasons.

1. This was only a test for lens sharpness rather than an exercise in precision of focusing.
2. I've mislaid the software for the Mamiya  :( and I'm not sure that it would work with windows 7 anyway.
Title: Re: MF Macro examples
Post by: Justinr on January 15, 2014, 12:26:05 pm
Just to give an idea of scale, I have a problem with lens sharpness rather than DOF it seems.
Title: Re: MF Macro examples
Post by: EricWHiss on January 15, 2014, 12:50:52 pm
I think you're onto something with the 120 lens as the 80mm shot did look better.  I'm not sure your 120 is up to spec.  However you might want to take a series of shots with the 120 while focus bracketing just to rule out any possible focusing issues.  Just find the place on the lens you think is correctly focused, back up a bit and take about 10 shots moving the focus in tiny steps.  If you don't get any shots as sharp as with the 80 then you'll know for sure. 
Title: Re: MF Macro examples
Post by: Pics2 on January 15, 2014, 01:25:59 pm
I think you're onto something with the 120 lens as the 80mm shot did look better.  I'm not sure your 120 is up to spec.  However you might want to take a series of shots with the 120 while focus bracketing just to rule out any possible focusing issues.  Just find the place on the lens you think is correctly focused, back up a bit and take about 10 shots moving the focus in tiny steps.  If you don't get any shots as sharp as with the 80 then you'll know for sure. 
But, shoot at f8 or f11 this time. I think even f16 is too much.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 15, 2014, 02:53:27 pm
Hi,

What I would do is a step by step study:

1) Shoot outdoors in sunlight using f/5.6, are your results sharp? If so your lens is OK! Else check your focusing.
2) Shoot indoors, still at aperture f/5.6. Are your images sharp? If so do you have a well defined plane of sharpness where you intended to be? If not investigate! You can have bad focus or vibrations.
3) Shoot with MLU and cable release. Hold a stead hand on the front of your lens during exposure? If holding hand on lens improves sharpness you have a vibration problem.
4) Now you can start stopping down, and see how aperture affect sharpness.

My take is really that I rather don't stop down beyond f/16. Keep also in mind that if you use extension, it makes the real aperture smaller. So f/16 at 1:1 is usually f/32.

I posted a diffraction test a while ago, it shows how image quality varies when stopping down with different amount of defocus. It was done on an APS-C camera but laws of optics are the same for all formats, but larger formats need less magnification. http://echophoto.dnsalias.net/ekr/index.php/photoarticles/49-dof-in-digital-pictures?start=1

Best regards
Erik


Just to give an idea of scale, I have a problem with lens sharpness rather than DOF it seems.
Title: Re: MF Macro examples
Post by: Justinr on January 15, 2014, 03:37:55 pm
I think you're onto something with the 120 lens as the 80mm shot did look better.  I'm not sure your 120 is up to spec.  However you might want to take a series of shots with the 120 while focus bracketing just to rule out any possible focusing issues.  Just find the place on the lens you think is correctly focused, back up a bit and take about 10 shots moving the focus in tiny steps.  If you don't get any shots as sharp as with the 80 then you'll know for sure.  

I went to do as you suggested using the ruler as a subject and lo and behold I was getting much sharper images. To explore this further I then chose a 'geometric' subject, ie, one with a defined shape and sharp edges, the dial on the old Pentax ESII in fact. Leaving the camera as it was I then replaced the Pentax with the twig and suddenly I was was back to the blur. However, note how much sharper the alloy mount (a hot shoe adapter) for the twig is compared to the mushy lichen.

NB, both are crops of a much larger image.

Title: Re: MF Macro examples
Post by: synn on January 15, 2014, 05:37:17 pm
You can download c1p7 demo and use it in db mode to do tethered shooting. It should work with your back.

I'd say do a tethered shoot to eliminate focusing errors and then check the files to see if the lens has a sharpness issue.
Title: Re: MF Macro examples
Post by: Justinr on January 16, 2014, 05:35:29 am
Just for comparison here's one taken on the Nikon D3, 24-85mm at 85mm.

I would say there is more definition in this shot but it is difficult to be sure without doing a Macro back to back comparison on the cameras. Could it not be the lens but the firmware that's causing the problems in the Mamiya ZD back? I might follow Synn's advice and see if a different RAW converter gives any improvement.

Edit. I found the Mamiya Capture Software and as I feared it doesn't seem to get on with Windows 7, it installs but won't open images.

Title: Re: MF Macro examples
Post by: EricWHiss on January 16, 2014, 10:52:28 am
I'm pretty sure at f/16 with your back, you'll still get usably sharp images and you'll need it since the DOF will be very thin otherwise.
In the comparison you posted of the pentax and lichen, it seems that neither subject is entirely within the DOF as both have areas of sharpness and softness. I'd say your DOF is only a few mm deep only.  This isn't enough to catch everything.


Have you tried backing away from your subject yet?  A farther camera to subject distance will increase the focus DOF (depth of field).   In the example's I posted, the one with the rollei 150mm the DOF isn't sufficient to cover the thickness of the medallion, but in the mamiya 120 samples you can see several inches of DOF - therefore backing away is one way to get your subject to be all included in DOF.    It means you have to crop down your file significantly, however you may end up with a more usable image.  


Title: Re: MF Macro examples
Post by: Justinr on January 16, 2014, 12:27:47 pm
I'm pretty sure at f/16 with your back, you'll still get usably sharp images and you'll need it since the DOF will be very thin otherwise.
In the comparison you posted of the pentax and lichen, it seems that neither subject is entirely within the DOF as both have areas of sharpness and softness. I'd say your DOF is only a few mm deep only.  This isn't enough to catch everything.


Have you tried backing away from your subject yet?  A farther camera to subject distance will increase the focus DOF (depth of field).   In the example's I posted, the one with the rollei 150mm the DOF isn't sufficient to cover the thickness of the medallion, but in the mamiya 120 samples you can see several inches of DOF - therefore backing away is one way to get your subject to be all included in DOF.    It means you have to crop down your file significantly, however you may end up with a more usable image.  




I think that I am getting somewhere now.

This was taken with the Mamiya 120mm lens at f16 and and about an inch further away. The big difference is though that it is a stack of 12 shots using Combine XP. I'm still not 100% happy but it's looking a lot better.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 16, 2014, 01:10:23 pm
Hi,

Is the crop you show actual pixels? What distance are you shooting at (distance from sensor plane to subject)?



Some ideas to improve your image:

- Have you tried different sharpening methods?
- You could repeat the experiment with f/8, even if I feel you should have good results at f/16

Best regards
Erik


I think that I am getting somewhere now.

This was taken with the Mamiya 120mm lens at f16 and and about an inch further away. The big difference is though that it is a stack of 12 shots using Combine XP. I'm still not 100% happy but it's looking a lot better.
Title: Re: MF Macro examples
Post by: synn on January 16, 2014, 08:06:03 pm
Extension tubes will also help to get closer to the subject without facing the issues you're facing now with close focusing.
Title: Re: MF Macro examples
Post by: EricWHiss on January 16, 2014, 08:56:01 pm
If you are willing to focus stack, then it's better to use a little bigger aperture - perhaps f/11 would be a good compromise between number of frames and sharpness for your setup.

Definitely you've made some improvements, but still I wonder if this is as sharp as you can get it? 
Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 17, 2014, 03:49:08 am
If you are willing to focus stack, then it's better to use a little bigger aperture - perhaps f/11 would be a good compromise between number of frames and sharpness for your setup.

Stacking is indeed a compromise between optimal quality with the lens used, and number of frames. One should first establish the best aperture that's possible for the magnification factor one requires. Since close-up focusing not only increases the magnification factor of our subject matter, it also magnifies the diffraction and defocus blur per pixel. That will lead to an optimal aperture that may be a bit wider than expected. Depending on the specific lens involved, an initial guess at 2 stops narrowed than wide open is often not too far off the mark.

That will lead to a DOF, based on a COC for the intended use of the image, and that will dictate the number of frames for stacking. 

Quote
Definitely you've made some improvements, but still I wonder if this is as sharp as you can get it?

I share your doubts.

Cheers,
Bart
Title: Re: MF Macro examples
Post by: Justinr on January 17, 2014, 04:07:09 am
So what we are saying is that if using focus stacking then we might as well rely on the software to provide DoF whilst trying to provide the sharpest material for it to work with? It sounds a sensible approach and I'll try it again with a much a wider aperture. Many thanks for the suggestions so far BTW.
Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 17, 2014, 04:47:41 am
So what we are saying is that if using focus stacking then we might as well rely on the software to provide DoF whilst trying to provide the sharpest material for it to work with?

Absolutely, assuming there are no other lens issues and the increased number of required frames is no objection.

As a tool to provide guidance with stacking, such as the number of frames needed to achieve a given output quality goal, you can use my free output quality planning tool (http://bvdwolf.home.xs4all.nl/main/foto/dofplan/dofplan.html), discussed here (http://www.luminous-landscape.com/forum/index.php?topic=81485.0).

Cheers,
Bart
Title: Re: MF Macro examples
Post by: EricWHiss on January 17, 2014, 07:57:17 pm
Total aside, but I've been doing a LOT of lens testing lately and while some MF lenses like the Rollei Schneider ones reach their peak sharpness at or near wide open, many others such as older Zeiss MF are still developing sharpness stopped further and further down even as they become affected by diffraction.  Like Bart suggests, knowing the behavior of your lens will help you decide what aperture to use.  If you shoot at f/8  you'll need more frames for stacking.

But I am still wondering if you can't get a 'workable' image backed off a fair bit, not just a few inches but a few feet back.  Your lichen sample may only fill but a fraction of the frame, but you may get the whole of it within DOF then in a single frame.   I'm not sure what your intended use is, but perhaps if you end up with a3000 pixel wide crop with a sharp image it would be sufficient for your use?

Title: Re: MF Macro examples
Post by: Phil Indeblanc on January 17, 2014, 11:57:12 pm
I would take the UV glass off the lens, if anything to at least rule it out.
Title: Re: MF Macro examples
Post by: Justinr on January 18, 2014, 11:00:44 am
Slight change of photographic subject just to try and get a handle on what's going on.

It's a dull afternoon so I went for f6.3 at 1/60th, ISO 200. Much above 200 and the ZD gets quite noisy and even at iso 50 noise can still be a problem which is why I'm not too keen on taking from a distance and then cropping right in, I fear it will exaggerate it. This shot is composed of 11 images.

There is something of a blur around the area of the clutch fluid reservoir which is more a software issue as one of the slices has that in focus.
Title: Re: MF Macro examples
Post by: EricWHiss on January 18, 2014, 11:38:08 am
What do you get of the bike with just one image instead of a stack?  And with the 80mm?    I'm still wondering about your 120 lens….
Title: Re: MF Macro examples
Post by: siebel on January 19, 2014, 03:41:49 am

 Keep also in mind that if you use extension, it makes the real aperture smaller. So f/16 at 1:1 is usually f/32.


Lest we create more confusion (Circles of? ???) here, this needs some clarification. For purposes of discussing diffraction, f/16 is f/16, regardless of focus distance or magnification. At 1:1 magnification, the amount of light coming through a given lens opening, say f/16, is spread over 4 times the area it would be covering compared to infinity focus. Thus, any given spot on your sensor is receiving 1/4 the illumination intensity, hence the 2stop loss. The key word here is intensity (remember, e=i x t). This has nothing whatsoever to do with how much diffraction you have at that aperture. So, when your lens is set to f/16 and your image magnification is 1:1, your illumination intensity or exposure is the equivalent of f/32(2-stops loss) but your diffraction remains what it would be at f/16.
Title: Re: MF Macro examples
Post by: Doug Peterson on January 19, 2014, 04:03:04 am
Lest we create more confusion (Circles of? ???) here, this needs some clarification. For purposes of discussing diffraction, f/16 is f/16, regardless of focus distance or magnification. At 1:1 magnification, the amount of light coming through a given lens opening, say f/16, is spread over 4 times the area it would be covering compared to infinity focus. Thus, any given spot on your sensor is receiving 1/4 the illumination intensity, hence the 2stop loss. The key word here is intensity (remember, e=i x t). This has nothing whatsoever to do with how much diffraction you have at that aperture. So, when your lens is set to f/16 and your image magnification is 1:1, your illumination intensity or exposure is the equivalent of f/32(2-stops loss) but your diffraction remains what it would be at f/16.

That's not correct.

Diffraction is based on effective aperture, not marked aperture (unless your camera system makes adjustments to account for bellows-loss for the displayed aperture).

This effect becomes quite obvious when dealing with very high magnification imaging. At 18:1 magnification diffraction on an 80mp back is meaningful starting at a lens marking of f/2.8 :).

Think of it from the perspective of the sensor: Here is the same sized physical opening, as seen with more and less extension:
(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-small-151x180.jpg)(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-large-151x180.jpg)

You can read more about this in my article on Extreme Macro Photography (http://www.doug-peterson.com/macro-extreme-technicals/).
Title: Re: MF Macro examples
Post by: siebel on January 19, 2014, 04:36:25 am
That's not correct.

Diffraction is based on effective aperture, not marked aperture (unless your camera system makes adjustments to account for bellows-loss for the displayed aperture).

This effect becomes quite obvious when dealing with very high magnification imaging. At 18:1 magnification diffraction on an 80mp back is meaningful starting at a lens marking of f/2.8 :).

Think of it from the perspective of the sensor: Here is the same sized physical opening, as seen with more and less extension:
(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-small-151x180.jpg)(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-large-151x180.jpg)

You can read more about this in my article on Extreme Macro Photography (http://www.doug-peterson.com/macro-extreme-technicals/).

You are right. I stand corrected. Thanks for jumping in!
Title: Re: MF Macro examples
Post by: Justinr on January 19, 2014, 07:48:06 am
Eric

I have attached the middle slice of the stack taken with the 120mm. I didn't have too much time yesterday to do much more than this image.


Doug

I've just addressed myself to your link and two things occurred to me.

1. Lighting. I note that you shoot in the dark to avoid shutter 'shake' but do you feel that extraneous ambient light or slight over exposure will reduce the clarity of the image? I slightly underexposed the bike shots to test this (although it's in no way a true comparison) for the brighter areas of the lichen shots appeared the least sharp.

2. You use a motion table to move the subject through the plane of focus, is this to avoid having to touch or disturb the camera or do you feel that there is some optical advantage over moving the plane of focus through the subject as I do by adjusting the lens?

Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 19, 2014, 09:50:50 am
2. You use a motion table to move the subject through the plane of focus, is this to avoid having to touch or disturb the camera [...]

That will potentially help, but,

Quote
[...]or do you feel that there is some optical advantage over moving the plane of focus through the subject as I do by adjusting the lens?

Optimal for stacking would be to leave the entrance pupil of the lens stationary with respect to the subject, and move the sensor plane for different focus planes. That will produce unchanged perspective between slices.

Second best depends on the ability to keep the entrance pupil as stationary as possible, probably by focusing the lens (if its focus throw allows such accurate and predictable setting). For really close-up work one can use a moving camera+lens bar, or use such a bar to move the subject (but try to have the lighting move along to avoid changing light directons).

Cheers,
Bart
Title: Re: MF Macro examples
Post by: EricWHiss on January 19, 2014, 11:47:17 am
Eric
I have attached the middle slice of the stack taken with the 120mm. I didn't have too much time yesterday to do much more than this image.

This looks more like the front of the stack than the middle to me. What on the bike do you consider to be your target?

Title: Re: MF Macro examples
Post by: ErikKaffehr on January 19, 2014, 12:22:34 pm
Hi,

Thanks for chiming in. Checked out your article and liked it.

I have still not found out the issue OP has, but it seems he made progress and that is a good thing.

Best regards
Erik


That's not correct.

Diffraction is based on effective aperture, not marked aperture (unless your camera system makes adjustments to account for bellows-loss for the displayed aperture).

This effect becomes quite obvious when dealing with very high magnification imaging. At 18:1 magnification diffraction on an 80mp back is meaningful starting at a lens marking of f/2.8 :).

Think of it from the perspective of the sensor: Here is the same sized physical opening, as seen with more and less extension:
(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-small-151x180.jpg)(http://www.doug-peterson.com/wordpress/wp-content/uploads/2009/08/Mamiya-AutoBellows-4-aperture-large-151x180.jpg)

You can read more about this in my article on Extreme Macro Photography (http://www.doug-peterson.com/macro-extreme-technicals/).
Title: Re: MF Macro examples
Post by: Justinr on January 19, 2014, 12:45:07 pm
Hi,

Thanks for chiming in. Checked out your article and liked it.

I have still not found out the issue OP has, but it seems he made progress and that is a good thing.

Best regards
Erik



I am indeed and it's all due to the kind help I am receiving here.

I have posted the latest attempt at the lichen below, the discs are approx 1mm in diameter.

This was taken with a stationary camera and fixed lens at f8 and under exposed by half a stop or so. The subject was moved through the focal plane using a jury rigged motion table of such crudity that I daren't mention its construction here.  :)  Anyway, 21 slices were taken at 0.8mm apart approx and this was the result of the stacking.

Edit. I've also attached an original slice.
Title: Re: MF Macro examples
Post by: Hank Keeton on January 19, 2014, 01:29:22 pm
Hopefully this is not too off-point for the OP, but  Doug, I'd like to congratulate you on your very systematic presentation and discussion of "Extreme" macro work. Your illustrations are immensely informative.

I'd be tempted to open a dialogue with you off-line if you like, so we don't distract from the OP's direction. I use a Hassy-555ELD with Zeiss-135+bellows, and the Sinar P2 with  SK Makro-Symmar-HM-80, Roden Macro-Sironar-120, SK Makro-Symmar-HM-180 (a superb lens in all aspects...), and Roden Macro-Sironar-N-300 (which requires an unbelievable amount of supplemental light at full extension!).

Your inclusion of the Velmex moving-table is brilliant, and one I'll pursue myself. Also, your discussion of diffraction+extension is a basic steep learning curve for the effective use of bellows. I certainly agree with your summary of stitching errors using Helicon Focus, and I'm impressed by your "several hundred images" successfully stitched! I've found I can work Helicon either closer-to-farther or the reverse, with about the same results. You're quite right about maintaining the continuity of sequencing!

And, last but not least, I appreciate your addition of the non-moving shutter, something I have not attempted with my Aptus-65, but will explore, now that I've seen your results.

All in all, a very informative expose on "extreme" macro creativity! Thank you.

Cheers,

Hank
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 19, 2014, 03:09:26 pm
Thanks, nice to hear!

Are you using bellows, extension tubes or something?

Best regards
Erik

I am indeed and it's all due to the kind help I am receiving here.

I have posted the latest attempt at the lichen below, the discs are approx 1mm in diameter.

This was taken with a stationary camera and fixed lens at f8 and under exposed by half a stop or so. The subject was moved through the focal plane using a jury rigged motion table of such crudity that I daren't mention its construction here.  :)  Anyway, 21 slices were taken at 0.8mm apart approx and this was the result of the stacking.

Edit. I've also attached an original slice.
Title: Re: MF Macro examples
Post by: EricWHiss on January 19, 2014, 06:28:37 pm
Justinr,
I'm just browsing casual snap shots I have taken with my Rolleiflex AFi-ii 12 with the same focal length you are using.   Looking at those, I am really thinking that you should be able to do a lot better than you are without even stacking.   I still think you'd do better to back off the subject some and try for a single frame.  

Here's a hand held no mirror up or anything shot with the 120mm on my camera with some crops.  This is with f/11  and a camera mounted Metz 54 flash.  Literally no work, and no adjustments in post.  I can only imagine that were I to have taken a tripod and used mirror up and stacked a dozen frames what this would look like.

Title: Re: MF Macro examples
Post by: EricWHiss on January 19, 2014, 06:42:36 pm
Here's another shot this time with a Rollei 6008AF body and the Hasselblad 528c back and 90mm lens at that terribly diffraction ridden f-stop of f/16.  This time on a tripod.
First full frame, then a 100% crop which you have to click and enlarge to see fully.   

My point here is that you can get plenty of detail if you back off enough to fit the subject within the DOF in a single frame. I also still wonder if you couldn't be getting sharper images.


Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 19, 2014, 07:36:14 pm
I have posted the latest attempt at the lichen below, the discs are approx 1mm in diameter.

Quote
Edit. I've also attached an original slice.

Hi Justin,

Indeed, I think we are being painfully confronted with Diffraction limited imaging, at high magnification factors.

By calculating the actual magnification factor, you'll be able to more accurately estimate the actual DOF and diffraction limitations, and required focus bracketing intervals/quantities. Even f/8 might prove to be too narrow to allow low diffraction blur levels.

Cheers,
Bart
Title: Re: MF Macro examples
Post by: Doug Peterson on January 19, 2014, 07:59:36 pm
1. Lighting. I note that you shoot in the dark to avoid shutter 'shake' but do you feel that extraneous ambient light or slight over exposure will reduce the clarity of the image? I slightly underexposed the bike shots to test this (although it's in no way a true comparison) for the brighter areas of the lichen shots appeared the least sharp.

"Macro" photography covers a lot of technical range.

My article was specific to macro magnifications in the ball park of 10:1. At such high magnifications and with so many frames required for the stack, ANY vibration is severely detrimental. I had to turn off the A/C, take shallow breaths, use a sturdy table (and not actually touch the table at any point) and work some zen in the way I actuated the linear slide.

At lower magnifications the requirements to reduce vibration are not nearly as onerous.


2. You use a motion table to move the subject through the plane of focus, is this to avoid having to touch or disturb the camera or do you feel that there is some optical advantage over moving the plane of focus through the subject as I do by adjusting the lens?

Both methods work, but moving the subject IMO becomes preferable as you get into very high magnification. There are pros and cons both optically, mathematically, and in practicality. For instance it's a lot easier to move the weight of a fly's eye forward and backward in even increments on a linear slide vs. moving a view camera on a linear slide, especially if its' pointed down at an angle. It's also difficult to turn the focus wheel on a large-extension view camera 100 times, in even increments, without ever accidentally bumping the camera slightly left or right. Remember that at the magnification I was shooting I had maybe 5000 pixels going across a 2mm area of subject matter, which means each pixel covered less than a micron of subject matter. ANY bump in the camera left or right, even lightly tapping on the highly-levered-due-to-extension rear standard would chance registration by several pixels.

For what you're doing I'd focus more on the basics - high shutter speed, sharp aperture, solid tripod, repeated practice to find the various pitfalls.
Title: Re: MF Macro examples
Post by: Doug Peterson on January 19, 2014, 08:26:22 pm
Hopefully this is not too off-point for the OP, but  Doug, I'd like to congratulate you on your very systematic presentation and discussion of "Extreme" macro work. Your illustrations are immensely informative.

I'd be tempted to open a dialogue with you off-line if you like, so we don't distract from the OP's direction. I use a Hassy-555ELD with Zeiss-135+bellows, and the Sinar P2 with  SK Makro-Symmar-HM-80, Roden Macro-Sironar-120, SK Makro-Symmar-HM-180 (a superb lens in all aspects...), and Roden Macro-Sironar-N-300 (which requires an unbelievable amount of supplemental light at full extension!).

Your inclusion of the Velmex moving-table is brilliant, and one I'll pursue myself. Also, your discussion of diffraction+extension is a basic steep learning curve for the effective use of bellows. I certainly agree with your summary of stitching errors using Helicon Focus, and I'm impressed by your "several hundred images" successfully stitched! I've found I can work Helicon either closer-to-farther or the reverse, with about the same results. You're quite right about maintaining the continuity of sequencing!

And, last but not least, I appreciate your addition of the non-moving shutter, something I have not attempted with my Aptus-65, but will explore, now that I've seen your results.

All in all, a very informative expose on "extreme" macro creativity! Thank you.

Glad you enjoyed it.

It was purely an academic self-challenge. I get to play with some cool gear and work with some stunningly good photographers and interface with some of the top engineers in the photo biz - so it's nice when I have time to write up something like this.

Nearly everything in the article was snagged from google research I did along the way. I wish I would have kept better track of that research so I could have given proper credit to the various techniques and suggestions. You know how that is though – one link leads to another to another and soon you have no idea where you read what.

The only two things I felt I "discovered"* was the pre-stack stacking in Helicon (doing small batches before combining those batches) and the no-vibration shutter-by-flash which is fairly unique to digital backs.

*and it wouldn't surprise me at all if a dozen people had done both of these things first; I just didn't happen across it in my research
Title: Re: MF Macro examples
Post by: EricWHiss on January 20, 2014, 02:39:51 am
I'm a big believer in keeping things as simple as possible.  I highly doubt that you'd need to be shooting in the dark or need to use even a tripod to get a sharp image of your lichen.  I shoot hand held macro all the time with my Rollei Hy6, and I don't use mirror up either because I need to focus on the subject.   

You may still decide to focus stack, but what's the good of doing all that work until you can consistently get single frames that are sharp at least in the slice of DOF.  I still don't see that in your images and have been scratching my head as to why.  I still wonder about your 120 lens.

Here's some more casual shots taken much closer in - hand held, no mirror lockup.  Note in these I used the Leaf Sensor flex square automatic crop. These are images of some lilies on my kitchen table.  I would guess the stamen were 2-3 mm across in width?  See the individual grains of pollen on the crop when expanded.   The shot with the 50mm componon lens shows the individual cells of the flower petal and a little pile of pollen grains.   When I walk around my kitchen the table shakes and the flowers move a bit. It's not a problem if you use a small flash as this freezes things quite effectively.

I'm not saying these images couldn't be sharper either.  Were I to have used mirror lockup, tripod, held my breath, etc. they could be sharper. What I'm saying is these casual shots of mine seem sharper than what you are getting with your tripod etc so I wonder if there is something else you could be doing?



Title: Re: MF Macro examples
Post by: EricWHiss on January 20, 2014, 02:46:26 am
One more image and crop of the same bunch of flowers but here one of the flower had gotten old and dropped out of the vase.
Title: Re: MF Macro examples
Post by: Justinr on January 21, 2014, 02:54:15 pm
One more image and crop of the same bunch of flowers but here one of the flower had gotten old and dropped out of the vase.

My apologies for dropping off the radar and not getting back to you all but have been busy elsewhere over the last couple of days.

Justin.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 21, 2014, 03:55:30 pm
Hi,

That's OK, but it would be interesting to know what magnification you have, that is how large is your subject?

That would help us to calculate effective aperture and perhaps give a better understanding of your shooting situation. I agree with Eric Hiss that better detail could be expected, but it is pretty impossible to judge if all parameters are not know. What is your shooting distance, measured from sensor plane?

Best regards
Erik

My apologies for dropping off the radar and not getting back to you all but have been busy elsewhere over the last couple of days.

Justin.
Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 21, 2014, 06:44:57 pm
What is your shooting distance, measured from sensor plane?

Hi Erik,

That won't allow to accurately determine the magnification factor, except for infinity focus. The only reliable way is to measure the size of a known object size (e.g. tape measure or calipers) as it is rendered on the sensor (e.g. 'x' sensels with a sensel pitch of y microns).

Cheers,
Bart
Title: Re: MF Macro examples
Post by: RobertJ on January 21, 2014, 09:18:44 pm
I think you should back up and just take some normal shots, and evaluate the plane of focus, even if it's not where you actually focused.  I mean, it shouldn't be that hard to get sharp detail.

EricW, the dollar shots you posted show f/20 being sharper than f/16.  Are you seeing the same thing?  Is there any reason why it doesn't just keep going downhill after f/16?  Or maybe I'm seeing things wrong...
Title: Re: MF Macro examples
Post by: EricWHiss on January 21, 2014, 10:15:03 pm
I think you should back up and just take some normal shots, and evaluate the plane of focus, even if it's not where you actually focused.  I mean, it shouldn't be that hard to get sharp detail.

EricW, the dollar shots you posted show f/20 being sharper than f/16.  Are you seeing the same thing?  Is there any reason why it doesn't just keep going downhill after f/16?  Or maybe I'm seeing things wrong...

Good Catch!  For some reason the sharpening was turned up to 511 on that one file and I didn't notice it.  I'm sure when I did the test I was playing with this to see if I could tighten it up and forgot to reset it.

Here it is again with normal sharpening.  But still that was pretty cool that f/20 could be looking pretty sharp with that 5.2um 80mp back right?

Title: Re: MF Macro examples
Post by: EricWHiss on January 21, 2014, 10:23:10 pm
ps. T-1000
I agree with you and have been suggesting to back up and try to get one good frame with the whole subject within DOF.

Erik K - have a look at Justin's picture of his set up, his comment about the lichen disk's being approx 1 mm across, and also the picture with the ruler.  You'll get an approx idea of magnification. Not that it really matters, anyhow, since magnification differences at this level is mostly going to impact the size of the DOF and not how sharp the sharp part of the DOF is. 

In every shot there should be one slice that's sharp. I'd suggest working on getting that part sharp before worrying about stacking or other stuff.  Also Justin you might be having focus issues. I'm still wondering about the 120.   Another thing to look at is your lighting.  Small flash units work great for macro work since they can be placed quite close to the subject and are relatively large in scale to tiny subjects.   The most important part is the flash duration is quite fast compared to studio strobes. This really helps mitigate camera or other vibration.

I use a pocket wizard on the camera and one on the flash to get the flash close to the subject and off camera for more interesting lighting.

Title: Re: MF Macro examples
Post by: ErikKaffehr on January 22, 2014, 12:58:15 am
Hi,

I don't know how the images he post are scaled and cropped. He posted a slice where DoF was pretty thin, and that may indicate large magnification.

I would also think that in a slice which contain a part that is in focus there would be optimum sharpness in some part.

Some other reflections, he perhaps could sharpen quite a bit more. Illumination can be flat and result in a soft image.

On reflection of my own tests, I feel that what is lost to diffraction can be regained in part, but fine low contrast structures will be lost, I rechecked the images you have posted, and checked out the f/8 and f/16 images. I took to the freedom to cut and paste from your postings. The f/8 image shows a paper structure while the f/16 image shows very little paper structure. I have just copied f/8 and f/16 image side by side. This is pretty much in line with what I would have expected from my own experience. I hope you don't mind that I use your images in this way, I feel it is proper use.

Best regards
Erik


ps. T-1000
I agree with you and have been suggesting to back up and try to get one good frame with the whole subject within DOF.

Erik K - have a look at Justin's picture of his set up, his comment about the lichen disk's being approx 1 mm across, and also the picture with the ruler.  You'll get an approx idea of magnification. Not that it really matters, anyhow, since magnification differences at this level is mostly going to impact the size of the DOF and not how sharp the sharp part of the DOF is.  

In every shot there should be one slice that's sharp. I'd suggest working on getting that part sharp before worrying about stacking or other stuff.  Also Justin you might be having focus issues. I'm still wondering about the 120.   Another thing to look at is your lighting.  Small flash units work great for macro work since they can be placed quite close to the subject and are relatively large in scale to tiny subjects.   The most important part is the flash duration is quite fast compared to studio strobes. This really helps mitigate camera or other vibration.

I use a pocket wizard on the camera and one on the flash to get the flash close to the subject and off camera for more interesting lighting.


Title: Re: MF Macro examples
Post by: Bart_van_der_Wolf on January 22, 2014, 05:07:42 am
On reflection of my own tests, I feel that what is lost to diffraction can be regained in part, but fine low contrast structures will be lost, I rechecked the images you have posted, and checked out the f/8 and f/16 images. I took to the freedom to cut and paste from your postings. The f/8 image shows a paper structure while the f/16 image shows very little paper structure.

Hi Erik,

And that is part of the problem at hand, but I suspect there is more (maybe a lens issue, maybe lighting, maybe camera vibration, etc.). Diffraction will lower the high spatial frequency MTF response more than for low spatial frequencies. High spatial frequencies already have lower MTF response, partly due to the sampling with area aperture sensels. That combined response starts to visibly reduce micro-contrast when the aperture is still relatively wide.

In practice, I notice it for high contrast micro-detail when the diffraction pattern exceeds 1.5x the sensel pitch. For a 9 micron sensel pitch, that happens at f/10 and narrower (smaller sensel pitches at proportionally smaller apertures). Combined with the relatively flat lighting, that dictates apertures that are (significantly) wider than f/10, at small magnification factors(!).

When we increase the magnification factor by focusing closer than infinity, we also increase the apparent diffraction blur diameter. At 1:1 we already need f/5.0 or wider to not affect visible sharpness of high contrast micro-detail, low contrast micro-detail will suffer a lot more already.

Cheers,
Bart

P.S. Here is an example of how the Diffraction to Sensel pitch diameter (marked as "Dsd") ratio influences the visibility of various levels of (micro-)contrast:
(http://bvdwolf.home.xs4all.nl/temp/OPF/DiffSpotDiameter.jpg) (http://bvdwolf.home.xs4all.nl/temp/OPF/DiffSpotDiameter.jpg)
Click on the image for actual 100% zoom size

Here is another (animated) representation of the DOF/resolution trade-off, starting at f/5.6 there is a visible reduction of the higher micro-contrast detail:
(http://bvdwolf.home.xs4all.nl/temp/OPF/DIFFvsDOF.gif)
Title: Re: MF Macro examples
Post by: EricWHiss on January 22, 2014, 12:04:14 pm
Don't forget that while my $2 bill shots were taken with the mamiya and 120mm lens, the Aptus ii 12 back used has 5.2um sensor pitch, but Justin's back has larger. I have noted diffraction starts to be noticeable between f/8 and f/11 on the 80mp backs, however its not that noticeable and some lose can be 'mitigated' through software.

My lily pictures are clearly affected by diffraction but still usable - they look quite fine a normal print sizes even though I present a 100% pixel peeping crop.
Title: Re: MF Macro examples
Post by: ErikKaffehr on January 22, 2014, 03:42:19 pm
Hi,

This is from an apertures series I made recently, left is f/8 and right is f/16, this is on 6.8 my pixels. The difference between f/8 and f/11 is not very noticable visually, but very obvious between f/8 and f/16.

An important factor is effective aperture, if Justin is shooting 1:1 which would correspond to 480 mm distance from sensor to subject on a 120 mm, effective aperture f/8 would be f/16, that is the cause of my interest for scale and shooting distance.

Best regards
Erik


Don't forget that while my $2 bill shots were taken with the mamiya and 120mm lens, the Aptus ii 12 back used has 5.2um sensor pitch, but Justin's back has larger. I have noted diffraction starts to be noticeable between f/8 and f/11 on the 80mp backs, however its not that noticeable and some lose can be 'mitigated' through software.

My lily pictures are clearly affected by diffraction but still usable - they look quite fine a normal print sizes even though I present a 100% pixel peeping crop.