I've just been through this issue with Phase One tech support. It does make a difference. Capture One has a - shall we say distinctive - file management architecture which is designed - in their minds - to make photographers' lives easy by organizing their workflow within Capture One in a logical manner. The basic idea is to keep everything together from any one photo shoot in one set of folders which can be ported all together with complete integrity, so that all your adjustments are carried with the images, and the raw images and their corresponding processed images remain carried together. In order to keep your life in Capture One easy and to benefit from all this togetherness and portability, you need to create a "Session" for your photoshoot before you download and import the images into the program. Then you import them into the newly created Session, which has its counterpart in the Pictures folder of your hard drive. Having done that, you can work-up each image, process it into a TIFF and keep it stored in the Output folder of C-1 for that Session, which is also in your hard drive uniquely grouped into that Session. For people - like me - who are accustomed to letting their file structure on their hard drives govern how they organize their images, this is a reversal of procedure - the program creates the structure and you comply with it. You don't have to, but it makes life with Capture One much easier to deal with. Downloading your flash card directly into C-1 from the start eliminates the steps of first downloading to files you create on your hard-drive and then re-importing them into the C-1 structure. I've worked it both ways and Phase One's recommendation to proceed as above is definitely preferable.