After reading your previous post, I found:
This image was taken at ISO 800 on a 50D
Do you use this ISO value?
If so, that may explain the noise. Your "zero" exposure level is underexposed (noisy).
If you take multiple exposures, you have to put ISO at low level (no or minimum amplification).
To get more light, the exposure have to be increased (you have to change shutter speed).
When you increase ISO, the signal is amplified, but the same thing is true for noise.
If you increase ISO, you get more noise. Noise may be acceptable for color image, but may be nasty on some channel (generally blue and/or red are more noisy).
Jacopo