These are 100% crops from two 15 minute exposures taken about 15 minutes apart (one in between). They are both with the Canon 5D Mark 2, ISO 400, 15 minutes, f5.6, Canon 17-40L, same DPP recipe for processing, yet there is a dramatic difference in resolved detail. The different color of light can be excluded because I have several others showing the same lack of sharpness under lighting more similar to the first (this one was used because the objects were of similar size and shot at a similar distance). The image on the left was the first in a series of four long exposures and is clearly the sharpest. The image on the right was the third.
I'm thinking there are four possible explanations:
1. A minute change to focus (although I'm sure both were focused at infinity) really made a huge difference in resolved detail (if this is the case I've got to figure out a consistent way to get the focus exact in the dark!!)
2. The first image is the only image that wasn't taken while the camera was working on a long exposure noise reduction for a different image in the background. I don't think that should matter but I guess maybe it could.
3. The sensor was beginning to heat up after nearly an hour of exposure and a similar amount of dark frame subtraction going on in the background.
4. Out of the four images the first is the only one where the tripod didn't shake at all (I have no way to prove this).
Anyway, what do you think? I have not had a chance to test this yet but when I do I will post the results. If I were guessing I'd say #1 or #4 or a combo of both...