the answer is a definite MAYBE.
assuming you've got a technically good image to start with, it will still depend on the subject and type of detail in the image, viewing distance, and your expectations
most of my wildlife shots are hand-held with a 20D and 100-400, normally at f8 and ISO 400 or 800 with fill flash where possible - when printed super B (13x19) on my 2200 up-rezzed in photoshop (bicubic smoother) at 240dpi, the prints withstand close inspection which is how i like to display them - i have not been able to crop equivalent to 16x20 and get satisfactory (to me) prints
on the other hand i believe many of these images could be printed 16x20 if displayed so not to invite close inspection. i've not played with other programs to up-rez or varying the dpi - there are vastly different opinions about up-rezzing, but i don't think there are big gains to be had one way or the other for wildlife images, but then this is a borderline situation and the sum of a bunch of small details can add up
although i think the 100-400 and 20D are well matched, better lenses will result in sharper images - my 300f4 with a 1.4x is just noticeably sharper and a 300f2.8 with 1.4x is quite noticeably sharper (subjectively i think this will make a bigger difference than the nth up-rez fiddle)
as Tim says, you'll have to try it and judge the results -- i have a touristic castle image printed super B from a Canon s400 which i would not have anticipated to be possible (and don't anticipate trying to repeat)